H. Vaysset*, C. Meers*, J. Cury, A. Bernheim, S.H. Sternberg. Evolutionary origins of archaeal and eukaryotic RNA-guided RNAmodification in bacterial IS110 transposons. bioRxiv, doi: 10.1101/2024.06.21.599552 (2024).

S. Tang, V. Conte*, D.J. Zhang*, R. Žedaveinytė, G.D. Lampe, T. Wiegand, L.C. Tang, M. Wang, M.W.G. Walker, J.T. George, L.E. Berchowitz, M. Jovanovic, S.H. Sternberg. De novo gene synthesis by an antiviral reverse transcriptase. Science, 386, eadq0876 (2024).

S. Tang, S.H. Sternberg. Genome editing with retroelements. Science 382, 370–371 (2023).

J.T. George, C. Acree, J.-U. Park, M. Kong, T. Wiegand, Y.L. Pignot, E.H. Kellogg, E.C. Greene, S.H. Sternberg. Mechanism of target site selection by type V-K CRISPR-associated transposases. Science 382, eadj8543 (2023).

M.W.G. Walker*, S.E. Klompe*, D.J. Zhang, S.H. Sternberg. Novel molecular requirements for CRISPR RNA-guided transposition. Nucleic Acids Res 51, 4519-4535 (2023).

C. Meers, H. Le, S.R. Pesari, F.T. Hoffmann, M.W.G. Walker, J. Gazelle, S. Tang, S.H. Sternberg. Transposon-encoded nucleases use guide RNAs to promote their selfish spread. Nature 622, 863–871 (2023).

D.R. Gelsinger, P.L.H. Vo, S.E. Klompe, C. Ronda, H. Wang, S.H. Sternberg. Bacterial genome engineering using CRISPR RNA-guided transposases. Nat Protoc, doi: 10.1038/s41596-023-00927-3 (2023).

G.D. Lampe*, R.T. King*, T.S. Halpin-Healy, S.E. Klompe, M.I. Hogan, P.L.H. Vo, S. Tang, A. Chavez, S.H. Sternberg. Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases. Nat Biotechnol, doi: 10.1038/s41587-023-01748-1 (2023).

G.D. Lampe, S.H. Sternberg. Novel recombinases for large DNA insertions. Nat Biotechnol 41, 471-472 (2023).

A. Nemudryi, A. Nemudraia, T. Wiegand, S.H. Sternberg, B. Wiedenheft. A viral “codebreaker” intercepts a host alarm. Cell Host Microbe 30, 1647-1648 (2022).

F.T. Hoffmann*, M. Kim*, L.Y. Beh*, J. Wang, P.L.H. Vo, D.R. Gelsinger, J. Thomas George, C. Acree, J.T. Mohabir, I.S. Fernández, S.H. Sternberg. Selective TnsC recruitment enhances the fidelity of RNA-guided transposition. Nature 609, 384–393 (2022).

S.E. Klompe, N. Jaber, L.Y. Beh, J.T. Mohabir, A. Bernheim, S.H. Sternberg. Evolutionary and mechanistic diversity of Type I-F CRISPR-associated transposons. Mol Cell 82, 616-628 (2022).

Older publications

P.L.H. Vo, C. Acree, M.L. Smith, S.H. Sternberg. Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing. Mobile DNA 12, 1-8 (2021).

P.L.H. Vo, C. Ronda, S.E. Klompe, E.E. Chen, C. Acree, H.H. Wang, S.H. Sternberg. CRISPR RNA-guided integrases for high-efficiency and multiplexed bacterial genome engineering. Nat Biotechnol 39, 480-489 (2021).

T.S. Halpin-Healy, S.E. Klompe, S.H. Sternberg*, I.S. Fernandez*. Structural basis of DNA targeting by a transposon-encoded CRISPR-Cas system. Nature 577, 271-274 (2020).

P. Cameron, M.M. Coons, S.E. Klompe, A.M. Lied, S.C. Smith, B. Vidal, P.D. Donohoue, T. Rotstein, B.W. Kohrs, D.B. Nyer, R. Kennedy, L.M. Bahn, C. Williams, M.S. Toh, M.J. Irby, L.S. Edwards, T. Künne, J. van der Oost, S.J.J. Brouns, E.M. Slorach, C.K. Fuller, S. Gradia, S.B. Kanner, A.P. May, S.H. Sternberg. Harnessing Type I CRISPR–Cas systems for human genome engineering. Nat Biotechnol, 37, 1471-1477 (2019).

S.E. Klompe, P.L.H. Vo, T.S. Halpin-Healy, S.H. Sternberg. Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration. Nature 571, 219-225 (2019).

Featured in Nature: New & Views

S.E. Klompe, S.H. Sternberg. Harnessing “A Billion Years of Experimentation”: The Ongoing Exploration and Exploitation of CRISPR–Cas Immune Systems. CRISPR J 2, 141–158 (2018).

J.S. Chen, Y.S. Dagdas, B.P. Kleinstiver, M.M. Welch, A.A. Sousa, L.B. Harrington, S.H. Sternberg, J.K. Joung, A. Yildiz, J.A. Doudna. Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature 550, 407–410 (2017).

Y. Dagdas*, J.S. Chen*, S.H. Sternberg, J.A. Doudna, A. Yildiz. A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9. Science Advances 3, eaao0027 (2017).

R.N. Jackson*, P.B. van Erp*, S.H. Sternberg, B. Wiedenheft*. Conformational regulation of CRISPR-associated nucleases. Curr Opin Microbiol 21, 110–119 (2017).

E.A. Boyle*, J.O.L. Andreasson*, L.M. Chircus*, S.H. Sternberg, M.J. Wu, C.K. Guegler, J.A. Doudna, W.J. Greenleaf. High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding. Proc Natl Acad Sci USA 114, 5461–5466 (2017).

D. Singh, S.H. Sternberg, J. Fei, J.A. Doudna*, T. Ha*. Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9. Nat Comm 7, 1–8 (2016).

S.H. Sternberg*, H. Richter*, E. Charpentier, U. Qimron. Adaptation in CRISPR-Cas systems. Mol Cell 61, 797–808 (2016).

S.H. Sternberg, B. LaFrance, M. Kaplan, J.A. Doudna. Conformational control of DNA target cleavage by CRISPR-Cas9. Nature 527, 110–113 (2015).

S. Redding, S.H. Sternberg, M. Marshall, B. Gibb, P. Bhat, C.K. Guegler, B. Wiedenheft, J.A. Doudna*, E.C. Greene*. Surveillance and processing of foreign DNA by the Escherichia coli CRISPR-Cas system. Cell 163, 854–865 (2015).

S.H. Sternberg, J.A. Doudna. Expanding the biologist’s toolkit with CRISPR-Cas9. Mol Cell 58, 568–574 (2015).

A.V. Wright*, S.H. Sternberg*, D.W. Taylor, B.T. Staahl, J.A. Bardales, J.E. Kornfeld, J.A. Doudna. Rational design of a split-Cas9 enzyme complex. Proc Natl Acad Sci USA 112, 2984–2989 (2015).

M.R. O’Connell, B.L. Oakes, S.H. Sternberg, A. East-Seletsky, M. Kaplan, J.A. Doudna. Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature 516, 263–266 (2014).

M.L. Hochstrasser*, D.W. Taylor*, P. Bhat, C.K. Guegler, S.H. Sternberg, E. Nogales, J.A. Doudna. CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference. Proc Natl Acad Sci USA 111, 6618–6623 (2014).

M. Jinek*, F. Jiang*, D.W. Taylor*, S.H. Sternberg*, E. Kaya, E. Ma, C. Anders, M. Hauer, K. Zhou, S. Lin, M. Kaplan, A.T. Iavarone, E. Charpentier, E. Nogales, J.A. Doudna. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science 343, 1247997-1–11 (2014).

S.H. Sternberg*, S. Redding*, M. Jinek, E.C. Greene, J.A. Doudna. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature 507, 62–67 (2014).

R.E. Haurwitz, S.H. Sternberg, J.A. Doudna. Csy4 relies on an unusual catalytic dyad to position and cleave CRISPR RNA. EMBO J 31, 2824–2832 (2012).

S.H. Sternberg, R.E. Haurwitz, J.A. Doudna. Mechanism of substrate selection by a highly specific CRISPR endoribonuclease. RNA 18, 661–672 (2012).

B. Wiedenheft, S.H. Sternberg, J.A. Doudna. RNA-guided genetic silencing systems in bacteria and archaea. Nature 482, 331–338 (2012).

S. Chakravarthy, S.H. Sternberg, C.A. Kellenberger, J.A. Doudna. Substrate-specific kinetics of Dicer-catalyzed RNA processing. J Mol Biol 404, 392–402 (2010).

J. Fei, J. Wang, S.H. Sternberg, D.D. MacDougall, M.M. Elvekrog, D.K. Pulukkunat, M.T. Englander, R.L. Gonzalez. A highly purified, fluorescently labeled in vitro translation system for single-molecule studies of protein synthesis. Meth Enzymol 472, 221–259 (2010).

S.H. Sternberg, J. Fei, N. Prywes, K.A. McGrath, R.L. Gonzalez. Translation factors direct intrinsic ribosome dynamics during translation termination and ribosome recycling. Nat Struct Mol Biol 16, 861–868 (2009).